Physiological basis of post-harvest ripening and standardization of seed extraction in ash gourd

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Published

2023-06-28

DOI:

https://doi.org/10.58993/ijh/2023.80.2.4
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Vol. 80 No. 2 (2023): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2023 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Nakul Gupta ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • P.M. Singh ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • Rajesh Kumar ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • Tribhuvan Chaubey ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • Vikas Singh ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India

Abstract

Manual seed extraction of ash gourd is time and labour extensive, and freshly harvested seeds possess dormancy. Hence, seed extraction protocol was standardized, and physio-biochemical changes associated with post-harvest ripening (PHR) were elucidated in ash gourd cv. Kashi Dhaval. Allowing pulp to ferment for 24 to 48 h gave the best quality seed (germination and vigour) compared to acid, alkali and manual extraction. PHR of fruit for 20-30 days before seed extraction gave the higher seed yield (seeds per fruit and 100-seed weight), higher seed germination and vigour compared to seed obtained from freshly harvested fruits. Physiological analysis showed that PHR increased the seed reserve (total soluble proteins, starch) except total soluble sugars, conceivably due to conversion to starch. Decreased O2 and H2 O2 activity in PHR seeds indicated a lower threat to oxidative stress, probably due to decreased seed moisture. Consequently, SOD, CAT and POD activity was reduced in PHR seeds. In conclusion, allowing fruit pulp to ferment for 24 to 48 h gave the best quality seed during seed extraction. Moreover, 20-30 days PHR in ash gourd is recommended because numerous physiobiochemical changes related to dormancy release and germination enhancement occur during PH

How to Cite

Gupta, N., Singh, P. M., Kumar, R., Chaubey, T., & Singh, V. (2023). Physiological basis of post-harvest ripening and standardization of seed extraction in ash gourd . Indian Journal of Horticulture, 80(2), 153–158. https://doi.org/10.58993/ijh/2023.80.2.4

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