An efficient protocol for shoot organogenesis and plant regeneration in jackfruit

Downloads

Published

2022-12-05

Keywords:

Artocarpus heterophyllus, micropropagation, BAP, GA3, plant growth regulators
Dimensions Badge

Issue

Vol. 79 No. 3 (2022): Indian Journal of Horticulture

Section

Research Articles

License

Copyright (c) 2022 Indian Journal of Horticulture

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Ratna Rai Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Divyangana Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Ranjan Srivastava Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Rajesh Kumar Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • V.P. Singh Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Pratibha Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India

Abstract

The present investigation was carried out for the rapid clonal mass multiplication of disease free plants of jackfruit cv. Pant Garima. The nodal explants were collected in winter season proved to be the best with regard to maximum survival of explants. The effect of sterilants (HgCl2 and ethanol) in various combinations was also assessed on survival percentage of explants. The MS medium was supplemented with five concentrations of BAP (1.0, 2.0, 3.0, 4.0 and 5.0 mgL-1) along with control (0.0mgL-1) and their effects on shootlets regeneration, and length of shootlets were evaluated. Maximum shoot proliferation was obtained in the medium supplemented with 4.0 mgL-1 BAP. Further, GA3 @ 1.0 mgL-1 added to MS medium supplemented with 4.0 mgL-1 BAP gave the best results for enhancing the length of the shootlets. For rooting, half strength of MS liquid medium fortified with 2.0 mgL1 IBA gave maximum rooting.

How to Cite

Rai, R., Divyangana, Srivastava, R., Kumar, R., Singh, V., & Pratibha. (2022). An efficient protocol for shoot organogenesis and plant regeneration in jackfruit. Indian Journal of Horticulture, 79(3). Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/483

Downloads

Download data is not yet available.

References

. Ali, J. and Feyissa, K. Bantte. 2000.Protocol

optimization for in vitro shoot multiplication of

jackfruit (Artocarpus heterophyllus L.). Afr. J.

Biotechnol. 16: 87-90.

Amany,M.H., Ali E.A.M. and Ehab, S.B. 2007. In

vitro propagation of jackfruit. J Appl. Sci. Res. 3:

-26.

Ashrafuzzaman, M., Kar, S., Khanam, D. and

Prodhan, S.H. 2012. In Vitro regeneration

and multiplication of jackfruit (Artocarpus

heterophyllusL.). Res. J. Biol. 02: 59-65.

Bandaralage, J.C.A.H., Hayward, A., O’Brien, C.

and Mitter, N. 2015. Gibberellin and cytokinins

in synergy for a rapid nodal multiplication

system of avocado. In: Actas. Proceedings:

Genetic Resources and Nursery Management.

pp. 95-103.

Bisht, Tejpal, Rawat, Laxmi and Singh, Pramod.

Effect of collection season, type and

length of explant on callus induction and its

survival in peach. J. Hill Agric.7. 215- 21.

Desjardins, Y., Dubuc, J. F. and Badr, A. 2009.

In vitro culture of plants: A stressful activity. Acta

Hortic. 812: 29–50.

Faisal, S.M., Forkan, M. A. and Amanullah, M.

Study of in vitro shoot production of offseason Jackfruit (Artocarpus heterophyllus Lam).

Int. J. Adv. Res. Inn. 9: 44-47.

Gonbad, R.A., Sinniah, U.R., Abdul Aziz, M.

and Mohamad, R. 2014. Influence of cytokinins

in combination with GA3

on shoot multiplication

and elongation of tea clone Iran 100 (Camellia

sinensis (L.) O. Kuntze). Sci. World J.: 9.https://

doi.org/10.1155/2014/943054.

Harb, M.H., Reda, M., Alhady, A.A. and Elsalam,

N.A.A. 2015. In vitro rapid propagation of

jackfruit (Artocarpus heterophyllus Lam.). Am.

Eurasian J. Agric. Environ. Sci. 15: 147-53.

Itoo, H., Syamal, M.M., Upadhyay, S., Ahuja,

P. and Mir, H. 2013. In vitro plant regeneration

of grape cv. Perlette through axillary bud and

shoot tip explants. Indian J. Hortic. 70: 185-89.

Khan, F.R., Ur-Rahman, H., Abbasi, N., Ibrahim,

M. and Abbas, G. 2010. In vitro shoot and root

proliferation of jack fruit as affected by different

concentrations of growth regulators. Sarhad J.

Agric. 26: 533-38.

Martini, A.N., Papafotiou, M. and Vemmos,

S.N. 2013. Season and explant origin affect

phenolic content, browning of explants, and

micropropagation of ×Malosorbus florentina

(zucc.) Browicz. HortSci., 48: 102–107.

Meghwal, P.R., Sharma, H.C. and Singh, S.K.

Micropropagation studies on guava.

Indian J. Hortic. 67: 55-58.

Miro, C.B. and Acedo, V.Z. 2015. Development

of micropropagation protocol supporting

sustainable production of jackfruit (Artocarpus

heterophyllus Lam.). Acta Hortic. 1088: 505-

Rai, R., and Misra, K.K. 2005. Micropropagation

of karonda (Carissa carandas) through shoot

multiplication. Sci.Hortic. 103: 227-32.

Saha, S., Mori, H. and Hattori, K. 2007.

Synergistic effect of kinetin and benzyl adenine

plays a vital role in high frequency regeneration

from cotyledon explants of bottle gourd

(Lagenaria siceraria) in relation to ethylene

production. Breed Sci. 57: 197-202.

Singh, A. 2003. Fruit Physiology and

Production. Kalyani Publishers. New Delhi.

p.

Sreeni, K.R. 2020. Jackfruit – Future food

security: A case study of Ayur Jack farm of

Thissur, Kerala. Int. J. Adv. Res. 8: 787–90.

Sulusoglu, M., and Cavusoglu, A. 2013.

Micropropagation of cherry laurel Prunus

laurocerasus L. J. Food Agric. Environ. 11: 576-

Swami, S. and Kalse, S. 2018. Jackfruit

(Artocarpus heterophyllus): Biodiversity,

Nutritional Contents, and Health. doi.

org/10.1007/978-3-319-54528-8_87-1.

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 > >>