Indirect somatic embryogenesis in sweet orange cv. “Mosambi”

Published

2023-03-27

DOI:

https://doi.org/10.58993/ijh/2023.80.1.4

Keywords:

Citrus sinensis L., epicotyl, callus, plantlets
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Authors

  • Reena Prusty Division of Fruits & Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, Delhi, India
  • O. P. Awasthi Division of Fruits & Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, Delhi, India
  • S.K. Singh Division of Fruits & Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, Delhi, India
  • C. Bharadwaj Division of Genetics, ICAR-IARI, New Delhi-110012, Delhi, India.

Abstract

This study was carried out to standardize a reliable protocol by using different explants (epicotyl, cotyledon, and root), plant bioregulators (PBRs), and carbon sources on indirect embryogenesis during 2019-2022. Among the treatments tested, T4 (MS + 2,4-D (1.5 mg L-1) + BAP (1.0 mg L-1) + ME (500 mg L-1)) proved best for callusing (90.89 %), callus fresh weight (0.83 g), turgid weight (0.84 g) and dry weight (0.08 g) as compared to others. In terms of callusing (83.72%), callus fresh weight (0.67 g), turgid weight (0.68 g), dry weight (0.05 g) and callus water content (11.28 %), explants epicotyl (E) showed its superiority over others. Similarly, the treatment combination T4 × E surpassed others in callusing potential. Embryogenesis (59.09%) and germination (33.61%) were best in T7 (MS + BAP (2 mg L-1) + NAA (0.1 mg L-1) + ME (500 mg L-1)). Among the different carbon sources, 5% glycerol supplemented with T7 proved best in inducing the highest number of somatic embryos/callus mass (73.26), embryogenesis (65.27%), and plantlet formation (68.77%). Therefore, the standardized protocol can be used for indirect embryogenesis for different genotypes of sweet orange.

How to Cite

Prusty, R., Awasthi, O. P., Singh, S., & Bharadwaj, C. (2023). Indirect somatic embryogenesis in sweet orange cv. “Mosambi”. Indian Journal of Horticulture, 80(1), 17–23. https://doi.org/10.58993/ijh/2023.80.1.4

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