Indirect somatic embryogenesis in sweet orange cv. “Mosambi”
Downloads
Published
DOI:
https://doi.org/10.58993/ijh/2023.80.1.4Keywords:
Citrus sinensis L., epicotyl, callus, plantletsIssue
Section
License
Copyright (c) 2023 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
This study was carried out to standardize a reliable protocol by using different explants (epicotyl, cotyledon, and root), plant bioregulators (PBRs), and carbon sources on indirect embryogenesis during 2019-2022. Among the treatments tested, T4 (MS + 2,4-D (1.5 mg L-1) + BAP (1.0 mg L-1) + ME (500 mg L-1)) proved best for callusing (90.89 %), callus fresh weight (0.83 g), turgid weight (0.84 g) and dry weight (0.08 g) as compared to others. In terms of callusing (83.72%), callus fresh weight (0.67 g), turgid weight (0.68 g), dry weight (0.05 g) and callus water content (11.28 %), explants epicotyl (E) showed its superiority over others. Similarly, the treatment combination T4 × E surpassed others in callusing potential. Embryogenesis (59.09%) and germination (33.61%) were best in T7 (MS + BAP (2 mg L-1) + NAA (0.1 mg L-1) + ME (500 mg L-1)). Among the different carbon sources, 5% glycerol supplemented with T7 proved best in inducing the highest number of somatic embryos/callus mass (73.26), embryogenesis (65.27%), and plantlet formation (68.77%). Therefore, the standardized protocol can be used for indirect embryogenesis for different genotypes of sweet orange.Abstract
How to Cite
Downloads
Similar Articles
- Manoj Kundu, A. K. Dubey, Anatomical studies of in vitro raised haploid and diploid citrus plants obtained through gamma irradiated pollen , Indian Journal of Horticulture: Vol. 79 No. 02 (2022): Indian Journal of Horticulture
- Dinesh Kumar, Lallan Ram, M. S. Ladaniya, Archana Khadse, Sunil Kumar, Environmental impact on biochemical parameters during developmental stages of Citrus fruit , Indian Journal of Horticulture: Vol. 76 No. 02 (2019): Indian Journal of Horticulture
- Lal Chand, Effect of rootstock and age of seedling on success of in vitro shoot tip grafting in Kinnow mandarin , Indian Journal of Horticulture: Vol. 73 No. 1 (2016): Indian Journal of Horticulture
- Anurag Kashyap, D.P. Deshmukh, S. Acharjee, K.K. Biswas, Prevalence of Citrus tristeza virus in North Eastern region of India and molecular characterization of its isolates , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture
- C.N. Rao, V.J. Shivankar, Shyam Singh, Oviposition preference of some insect pests of citrus in relation to leaf/ twig age of Nagpur mandarin , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- R.A. Marathe, A.A. Murkute, M.S. Ladaniya, R.K. Sonkar, Jayashree Kolwadkar, Standardization of potting media and nutrition protocol for raising acid lime seedling in containerized nursery , Indian Journal of Horticulture: Vol. 77 No. 02 (2020): Indian Journal of Horticulture
- Raj Kumar, M.K. Kaul, S.N. Saxena, S. Bhargava, S.S. Sirowa, Acclimatization of in vitro generated Citrus plantlets , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- Anuradha ., S.K. Sehrawat, D.S. Dahiya, Assessment of genetic integrity of plants regenerated by organogenesis from callus culture of strawberry , Indian Journal of Horticulture: Vol. 74 No. 01 (2017): Indian Journal of Horticulture
- A.K. Goswami, A.K. Dubey, A.K. Singh, S.K. Singh, Manish Srivastav, Jai Prakash, O.P. Awasthi, Kanhaiya Singh, Suneha Goswami, Effect of polyamines on physio-chemical and biochemical parameters of citrus rootstocks under NaCl stress , Indian Journal of Horticulture: Vol. 73 No. 04 (2016): Indian Journal of Horticulture
- Mohan Ram, K.V. Prasad, T. Janakiram, S.K. Singh, Ajay Arora, Callus induction and proliferation from Rosa hybrida leaf explants , Indian Journal of Horticulture: Vol. 72 No. 03 (2015): Indian Journal of Horticulture
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- S.K. Dutta, V.B. Patel, S.K. Singh, A.K. Singh, Adaptation of arbuscular mycorrhizal fungi inoculated Jatti khatti (Citrus jambhiri) seedlings under water deficit stress conditions , Indian Journal of Horticulture: Vol. 72 No. 04 (2015): Indian Journal of Horticulture
- P. Suresh Kumar, V.R. Sagar, S.K. Singh, Effect of ripening stages on physico-chemical characteristics of fresh and osmo-dehydrated aonla cv. Chakkaiya segments , Indian Journal of Horticulture: Vol. 70 No. 04 (2013): Indian Journal of Horticulture
- S. Jayaprahasam, Room Singh, S.K. Singh, Effect of nutrients sprays on fruit set and retention in mango post hybridization , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- H.R. Mahure, M.L. Choudhary, K.V. Prasad, S.K. Singh, Mutation in chrysanthemum through gamma irradiation , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- A.K. Goswami, A.K. Singh, A.K. Dubey, O.P. Awasthi, Jai Prakash, S.K. Singh, Kanhaiya Singh, Akshay Sakhare, Effect of elevated CO2 levels in Kinnow mandarin and Kagzi Kalan lemon under controlled environment conditions , Indian Journal of Horticulture: Vol. 72 No. 04 (2015): Indian Journal of Horticulture
- Tanushree Sahoo, U.M. Grace, Khalil Fitrat, Chavlesh Kumar, V.B. Patel, S.K. Singh, C. Bharadwaj, V.K. Sharma, Madhubala Thakre, A.K. Dubey, M.K. Verma, A.I. Mir, Preharvest application of methyl jasmonate for improving postharvest quality of ‘Pusa Navrang’ grapes , Indian Journal of Horticulture: Vol. 76 No. 04 (2019): Indian Journal of Horticulture
- Md. Jameel Jhalegar, R.R. Sharma, S.K. Singh, Effect of surface coatings on postharvest quality of Kinnow mandarin , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture
- S.K. Singh, BOOK REVIEW , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture
- S.K. Verma, S.K. Singh, Hare Krishna, V.B. Patel, Comparative performance of different grafting techniques in grape cv. Pusa Urvashi , Indian Journal of Horticulture: Vol. 69 No. 01 (2012): Indian Journal of Horticulture
- J.K. Ranjan, A.K. Chakrabarti, S.K. Singh, Pragya ., Studies on in vitro seed germination of chilli , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture