Effect of microbial-inoculants on growth and biochemical parameters of mango plantlets during bio-hardening

Published

2022-10-13

DOI:

https://doi.org/10.5958/0974-0112.2017.000008.1

Keywords:

Bio-hardening, in vitro in-ovulo embryo culture, mango, mycorrhizal inoculation.
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Authors

  • Ankit Singh Division of Fruits and Horticultural Technology, ICAR- Indian Agricultural Research Institute, New Delhi 110 012
  • Manish Srivastav Division of Fruits and Horticultural Technology, ICAR- Indian Agricultural Research Institute, New Delhi 110 012.
  • S.K. Singh Division of Fruits and Horticultural Technology, ICAR- Indian Agricultural Research Institute, New Delhi 110 012.
  • Shiv Lal Division of Fruits and Horticultural Technology, ICAR- Indian Agricultural Research Institute, New Delhi 110 012.

Abstract

The present experiment was conducted to investigate the effect of chemical mutagen on biological parameters such as seed germination, shoot and root length and to fix the lethal dose (LD50) of EMS in short day Indian onion cultivar Bhima Dark Red. Presoaked seeds of onion were treated with different doses (0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 % v/v) of Ethyl Methane Sulfonate. The optimal mutagenic doses were calculated using Probit Analysis based on germination percentage which were considered useful in developing onion mutagenized population to create genetic variability for various qualitative and quantitative traits. Seed germination was recorded after 7 and 12 days of sowing, while seedling parameters such as seedling height, dry and fresh weight of seedlings were taken after 12 days. Data revealed that the mutagenic agent significantly reduced seed germination and seedling growth parameters in terms of shoot and root length as compared to control. Significant reduction in the seed germination percent with the enhancement of concentration of EMS dose was exhibited.

How to Cite

Singh, A., Srivastav, M., Singh, S., & Lal, S. (2022). Effect of microbial-inoculants on growth and biochemical parameters of mango plantlets during bio-hardening. Indian Journal of Horticulture, 74(01), 20–26. https://doi.org/10.5958/0974-0112.2017.000008.1

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