Assessment of genetic integrity of plants regenerated by organogenesis from callus culture of strawberry

Abstract

The nodal segments of strawberry cultivar Ofra were cultured on MS medium supplemented with different concentrations of auxins and cytokinins for indirect regeneration via callus formation. MS medium supplemented with 4.0 mg/l NAA and 2.0 BAP yielded the highest percentage of callus. The highest response of shoot regeneration was obtained in MS medium fortified with 2.0 mg/l kinetin and 0.5 mg/l IAA for regeneration of shoots from callus. The regenerated shootlets were rooted on half-strength MS medium. The plantlets thus developed were hardened successfully. Some morphological variations were found in plants generated by callus. On the basis of variations, in vitro raised plantlets were subjected to evaluation of genetic uniformity using RAPD markers. Out of 21 primer screened, six primers showed amplification and the RAPD profiles obtained were found to be reproducible and monomorphic. Results revelaed that tissue culture raised plants of strawberry were genetically identical and clonally uniform.