Preliminary screening of citrus hybrids for identifying tolerance to Phytophthora nicotianae

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Published

2024-03-30

DOI:

https://doi.org/10.58993/ijh/2024.81.1.5

Keywords:

Citrus, in vitro leaf inoculation, hybrids, necrosis, Phytophthora tolerance
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Vol. 81 No. 01 (2024): Indian Journal of Horticulture

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Articles

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Copyright (c) 2024 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Harsimran Singh Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India
  • Anita Arora Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India
  • Krishan Kumar Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India

Abstract

Phytophthora is one of the most devastating pathogens affecting citrus production. Breeding resistant genotypes is a durable strategy to manage this pathogen. In this study, in vitro screening of 31 citrus hybrids was done against Phytophthora nicotianae using detached leaf assay. The hybrids tested were developed in twoway crosses between Rangpur lime (RL, Phytophthora sensitive) and sour orange (SO, Phytophthora tolerant). The relative Phytophthora sensitivity was determined from the necrotic lesion area after three days of pathogen inoculation. The parents and hybrids could be differentiated into five resistance classes on the basis of necrotic lesion size and area. Among the 18 SO × RL (SR) hybrids, three hybrids viz. SR 3, SR 10 and SR 19 exhibited the least leaf necrotic length (2.8-3.7 mm), breadth (3.2-3.9 mm) and area (9.2-15.0 mm2), indicating a highly resistant reaction. From the 13 RL × SO (RS) hybrids, RS 6 and RS 22 showed highly resistant reaction by virtue of the least necrotic area (12.7-17.9 mm2). Twelve SR and four RS hybrids produced necrotic lesions of >216.8 mm2 area and were rated as highly susceptible in this assay. The parents- Rangpur lime and sour orange recorded highly susceptible and resistant reaction, respectively, which authenticated the results of the leaf assay. The five highly resistant hybrids identified in this study after ex vitro validation of Phytophthora tolerance should prove helpful in commercial citriculture.

How to Cite

Singh, H., Arora, A., & Kumar, K. (2024). Preliminary screening of citrus hybrids for identifying tolerance to Phytophthora nicotianae. Indian Journal of Horticulture, 81(01), 32–36. https://doi.org/10.58993/ijh/2024.81.1.5

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Author Biographies

Harsimran Singh, Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India

M.Sc. student

Krishan Kumar, Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India

Senior Plant Pathologist (Fruits)

References

Aboutorabi, M. 2018. A review on the biological control of plant diseases using various microorganisms. J. Res. Med. Dent. Sci. 6: 30-35.

Ajengui, A., Bertolini, E., Ligorio, A., Chebil, S., Ippolito, A. and Sanzani, S.M. 2018. Comparative transcriptome analysis of two citrus germplasms with contrasting susceptibility to Phytophthora nicotianae provides new insights into tolerance mechanisms. Plant Cell Rep. 37: 483-99.

Aliya, S.S.S., Nusaibah, S.A., Mahyudin, M.M., Yun, W.M. and Yusop, M.R. 2022. Colletotrichum siamense and Pestalotiopsis jesteri as potential pathogens of new rubber leaf spot disease via detached leaf assay. J. Rubber Res. 25: 195-212.

Cacciola, S.O., Gilardi, G., Faedda, R., Schena, L., Pane, A., Garibaldi, A. and Gullino, M.L. 2020. Characterization of Colletotrichum ocimi population associated with black spot of sweet basil (Ocimum basilicum) in Northern Italy. Plants 9: 654.

Chen, J., Shen, Y., Chen, C. and Wan, C. 2019. Inhibition of key citrus postharvest fungal strains by plant extracts in vitro and in vivo: A review. Plants 8: 26.

Das, A.K., Nerkar, S., Kumar, A. and Bewage, S. 2016. Detection identification and characterization of Phytophthora spp. infecting citrus in India. J. Plant Pathology 98: 55-69.

Denman, S. and Sadie, A. 2001. Evaluation of a stem inoculation technique for assessing resistance to Phytophthora cinnamomi in Leucospermum cultivars. Australas Plant Pathol. 30: 11-16.

Graham, J.H. 1990. Evaluation of tolerance of citrus rootstocks to Phytophthora root rot in chlamydospore-infested soil. Plant Dis. 74: 743-46.

Graham, J. H. and Menge, J. A. 1999. Root health: Fungal diseases. In: Citrus health management. L. W. Timmer & L. W. Duncan (Eds.). St. Paul, Minnesota: American Phytopathological Society Press, pp. 126-35.

Herewini, E.M., Scott, P.M., Williams, N.M. and Bradshaw, R.E. 2018. In vitro assays of Phytophthora agathidicida on kauri leaves suggest variability in pathogen virulence and host response. N. Z. Plant Prot. 71: 285-88.

Kaur, Y. 2019. Characterization of Phytophthora species associated with citrus foot rot and its management in Punjab. Ph.D. dissertation, Punjab Agricultural University, Ludhiana, India

Lima, R.P.M., Maximo, H.J., Merfa, M.V., Dalio, R.J.D., Cristofani-Yaly, M. and Machado, M.A. 2018. Genetic tools and strategies for citrus breeding aiming at resistant rootstocks to gummosis disease. Trop. Plant Path. 43: 279-88.

Liu, Y., Heying, E. and Tanumihardjo, S.A. 2012. History, global distribution, and nutritional importance of citrus fruits. Compr. Rev. Food Sci. F. 11: 530-45.

Naqvi, S.A.M.H. 2002. Fungal diseases of citrus: Diagnosis and management. Tech. Bull. 5, ICAR-NRC for Citrus, Nagpur, India, p. 61.

Panabieres, F., Ali, G.S., Allagui, M.B., Dalio, R.J.D., Gudmestad, N.C., Kuhn, M.L., Roy, S.G., Schena, L. and Zampounis, A. 2016. Phytophthora nicotianae diseases worldwide: new knowledge of a long recognized pathogen. Phytopathol. Mediterr. 55: 20-40.

Rajput, N.A., Atiq, M., Tariq, H., Saddique, W.M. and Hameed, A. 2020. Citrus Gummosis: A Formidable challenge to citrus industry: A Review. Int. J. Biosci. 16: 131-44.

Savita, G.S.V. and Nagpal, A. 2012. Citrus diseases caused by Phytophthora species. GERF Bull. Biosci. 3: 18-27.

Twizeyimana, M., Ojiambo, P.S., Tenkouano, A., Ikotun, T. and Bandyopadhyay, R. 2007. Rapid screening of Musa species for resistance to black leaf streak using in vitro plantlets in tubes and detached leaves. Plant Disease 91: 308-14.

Yan, H., Zhong, Y., Jiang, B., Zhou, B., Wu, B. and Zhong, G. 2017. Guanggan (Citrus reticulata) shows strong resistance to Phytophthora nicotianae. Sci. Hortic. 225: 141-49.

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