In-vitro propagation of virus tolerant rootstock Carrizo citrange

Abstract

Studies on in-vitro propagation of virus tolerant rootstock Carrizo citrange were carried out using nodal segments of nucellar seedlings. Murashige and Skoog (1962) medium supplemented with BAP, kinetin for shoot proliferation and NAA and IBA for rooting were used in different concentration in alone or in combinations. Amongst for rooting BAP and kinetin, 1.0 mgl-1 BAP was found better. BAP was found better cytokinin in respect to reducing time needed to bud break and longer shoot length as compared to kinetin. Kinetin was superior cytokinin than BAP in respect to per cent survival and shoot length. In case of BAP with kinetin combinations maximum number of shoot (9.30) was ontained on BAP 2.0 mgl-1 + kinetin 0.5 mgl-1 and shoot length (2.50 cm) on BAP 0.5 mgl-1 + kinetin 1.0 mgl-1. NAA was found as better auxin in comparison to IBA for rooting of microshoots. Interaction of NAA with IBA, the minimum time taken to root induction (17.40 days) was recorded on NAA 0.1 mgl-1 + IBA 0.5 mgl-1, maximum number of roots (9.60) on NAA + IBA (0.5 mgl-1 each) and root length (7.10 cm) on NAA + IBA (1.0 mgl-1 each) were recorded. The rooted plantlets were successfully acclimatized in greenhouse, in pot containing soil, perlite and vermiculite in equal proportions. About 83.00 per cent success was recorded after 60 days of transfer from culture room.