Studies on mutagenesis in garlic using chemical mutagens to determine lethal dose (LD50) and create variability

Published

2015-06-25

Keywords:

Colchicine, ethyl methane sulphonate, garlic, mutation, LD50, sodium azide.
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Authors

  • Vijay Mahajan ICAR-Directorate of Onion & Garlic Research, Rajgurunagar, Pune 410 505, Maharashtra
  • Asha Devi ICAR-Directorate of Onion & Garlic Research, Rajgurunagar, Pune 410 505, Maharashtra
  • Anil Khar ICAR-Directorate of Onion & Garlic Research, Rajgurunagar, Pune 410 505, Maharashtra
  • K.E. Lawande ICAR-Directorate of Onion & Garlic Research, Rajgurunagar, Pune 410 505, Maharashtra

Abstract

A study was undertaken to determine the LD50 in garlic variety G-41, to create variability for desirable traits using sodium azide (SA), colchicine and ethyl methane sulphonate (EMS). Half cut garlic cloves after treating for 6 and 12 h, were planted in randomized block design. Among the durations of treatments, concentrations required to attain LD50 was higher when treated for 6 hours as compared to 12 h in all the three mutagens. LD50 in SA can be obtained at 0.021 and 0.024%, in colchicine at 0.058 and 0.097% and in EMS at 0.674 and 1.222% concentration in 12 h treatment, whereas, it can be obtained at 0.095 and 0.108% in SA, 0.124 and 0.145% in colchicine and 0.826 and 1.222% in EMS in 6 h treatment, respectively at the time of harvesting and at 45 days after planting. Same concentrations can be tried in other varieties or lines of garlic to create variability for further selection.

How to Cite

Mahajan, V., Devi, A., Khar, A., & Lawande, K. (2015). Studies on mutagenesis in garlic using chemical mutagens to determine lethal dose (LD50) and create variability. Indian Journal of Horticulture, 72(02), 289–292. Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/1530

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