Microsatellite and RAPD analysis of grape (Vitis spp.) accessions and identification of duplicates/misnomers in germplasm collection

Abstract

Microsatellite and RAPD primers were used to analyze forty four grape accessions from the National Grape Germplasm Repository, Pune, India. Forty four microsatellite primers generated 433 and were more informative than RAPD primers. Thirty three RAPD primers resulted in 420 bands among 44 accessions and generated unique bands for several accessions. The dendrograms of genetic relationship obtained with two classes of markers were comparable. Microsatellite analysis of this set of accessions identified six duplicates and misnomers and genetic identity of duplicates was established. This study will help in better germplasm management and for devising strategies for identifying core selection.