Effect of genotype, explant and culture medium on organogenesis in brinjal
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Egg plant, plant regeneration, organogenesis, shoot inductionIssue
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Abstract
Brinjal were regenerated from callus derived from hypocotyl, cotyledon and root explants of five genotypes, namely Punjab Barsati, Punjab Sadabahar, Jamuni Gola, PBSR-11 and BB-93C on MS medium containing different concentrations of IAA and BAP. A combination of 2.5 mg/l IAA + 0.5 mg/l BAP was found optimum for adventitious shoot induction from all explants. Genotype, explant and genotype x explant interaction showed highly significant effects on organogensis. Among genotypes, PBSR-11 showed maximum response for organogenesis (79.43%). However, among explants, cotyledon was significantly better than hypocotyl and root. Plants regenerated via adventitious shoots were rooted on half-strength MS basal medium in vitro.
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