Effect of genotype, explant and culture medium on organogenesis in brinjal

Abstract

Brinjal were regenerated from callus derived from hypocotyl, cotyledon and root explants of five genotypes, namely Punjab Barsati, Punjab Sadabahar, Jamuni Gola, PBSR-11 and BB-93C on MS medium containing different concentrations of IAA and BAP. A combination of 2.5 mg/l IAA + 0.5 mg/l BAP was found optimum for adventitious shoot induction from all explants. Genotype, explant and genotype x explant interaction showed highly significant effects on organogensis. Among genotypes, PBSR-11 showed maximum response for organogenesis (79.43%). However, among explants, cotyledon was significantly better than hypocotyl and root. Plants regenerated via adventitious shoots were rooted on half-strength MS basal medium in vitro.