Development of molecular marker for nitrate reductase (NR) gene to improve nitrogen use efficiency in potato

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Published

2019-03-13

DOI:

https://doi.org/10.5958/0974-0112.2019.00012.4

Keywords:

Solanum tuberosum, Cloning, CAPS marker, marker-assisted selection, nitrogen use efficiency.
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Issue

Vol. 76 No. 01 (2019): Indian Journal of Horticulture

Section

Articles

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Copyright (c) 2019 Indian J. Hortic.

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Jagesh K. Tiwari ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Rasna Zinta ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Vinod Kumar ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Devendra Kumar ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Tanuja Buckseth ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • RK Singh ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Shashi Rawat ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • SK Chakrabarti ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh

Abstract

Two diverse potato varieties Kufri Jyoti (N inefficient) and Kufri Gaurav (N efficient) were used to amplify, clone and analyze the sequence of nitrate reductase (NR) gene, involved in N metabolism in plants. Only single, distinct and un-fractioned two fragments amplified by the NR gene-based primers were cloned and sequence analyzed. Following sequence analysis, non-redundant sequences with uninterrupted open reading frames of NR homologues were identified to the known N metabolism. A cleaved amplified polymorphic sequence (CAPS) marker of the NR gene was developed for marker-assisted selection (MAS) in potato. The CAPS marker was validated in a tetraploid mapping population of 123 individuals and 48 potato varieties for its polymorphism. Thus, the development of new CAPS marker would be useful for MAS with better nitrogen use efficiency (NUE) in potato.

How to Cite

Tiwari, J. K., Zinta, R., Kumar, V., Kumar, D., Buckseth, T., Singh, R., … Chakrabarti, S. (2019). Development of molecular marker for nitrate reductase (NR) gene to improve nitrogen use efficiency in potato. Indian Journal of Horticulture, 76(01), 80–85. https://doi.org/10.5958/0974-0112.2019.00012.4

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