Standardization of in vitro mass multiplication protocol for hybrid tea rose cv. Grand Gala
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Rose, micropropagation, in vitro regenerationIssue
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Abstract
An efficient protocol for in vitro multiplication of hybrid tea rose cv. Grand Gala was developed using axillary bud segments. Out of different pre-treatments of explants tried, the highest explant survival (82.16%) was obtained with carbendazim (0.2%) + diathane M-45 (0.2%) + 8-HQC (200 mg/l) for 4 h on a horizontal shaker (120 rpm). Sucrose concentration of 40 g/l in the medium seems to be optimal for in vitro shoot multiplication. Murashige and Skoog medium supplemented with 5.0 mg /l BAP + 0.2 mg/l NAA + 0.5 mg/l GA3 was found most effective for culture establishment and shoot proliferation with highest number of micro-shoots (6.49 shoots / explant). Rooting of micro-shoots was induced on half-strength MS basal medium supplemented with NAA (0.5 mg/l) + IBA (0.5 mg/l). The regenerated plantlets were efficiently hardened in glass jars filled with vermiculite + agropeat (1:2) moistened with half-strength MS medium salts and covered with polypropylene lids, thereafter plants were successfully transferred to the glasshouse with good survival.
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