Effect of abscisic acid on anthocyanin production in callus culture of Petunia hybrida cv. Bravo Blue
Downloads
Published
Keywords:
Elicitors, Petunia hybrida, callus culture, abscisic acid, anthocyanin.Issue
Section
License
Copyright (c) 2014 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Development of an efficient tissue culture system for commercial production of anthocyanin requires an integrated approach combining the effects of various enhancement strategies. To develop a method which does not require the complicated operation and results in the anthocyanin induction in callus cultures of Petunia hybrida, we proposed a convenient culture method using Murashige and Skoog medium supplemented with different concentrations of Abscisic acid (ABA), viz., 0 (control), 20, 30, 40 and 50 μM and the callus cultured on it. Supplementation of 30 μM resulted in a synergistic increase in anthocyanin production in terms of pigment content and pigment production with highest response coefficient (91.29 ± 2.07%) in shortest duration (12.87 ± 0.18) days. Least response coefficient (65.55 ± 2.94%) was recorded under control treatment. The colour value index (CV) was observed maximum with the addition of ABA 30 μM (1.58 ± 0.08 CV g-1 FCW) and maximum response for pigment production (3.22 ± 0.12b CV test tube-1). Significant reduction in the callus growth was observed due to addition of ABA as it was maximum in control treatment (3.15 ± 0.70 g). Anthocyanin pigmentation was observed as bluish-purple dots scattered over the callus.Abstract
How to Cite
Downloads
Similar Articles
- Mohan Ram, K.V. Prasad, T. Janakiram, S.K. Singh, Ajay Arora, Callus induction and proliferation from Rosa hybrida leaf explants , Indian Journal of Horticulture: Vol. 72 No. 03 (2015): Indian Journal of Horticulture
- Meenu Kumari, S.K. Singh, Kishan Swaroop, Deeba Kamil, K.P. Singh, Diversity analysis of Fusarium oxysporum f. sp. gladioli isolates using Fatty Acid Methyl Ester (FAME) profiling , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- Hadi Ghasemi, Mehdi Rezaei, Hamid-Reza Asghari, Hassan Ghorbani-Ghouzhdi, Amin Sharififar, Cultivation of tuberose in pot and field with humic acid treatments under a semi-arid climate , Indian Journal of Horticulture: Vol. 73 No. 03 (2016): Indian Journal of Horticulture
- Prativa Lakhotia, Krishan Pal Singh, S.K Singh, M.C Singh, K.V Prasad, Kishan Swaroop, Influence of biotic and abiotic elicitors on production of betalain pigments in bougainvillea callus cultures , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- M.S. Aneesa Rani, S. Chandrasekaran, M. Vijayakumar, In-vitro production of secondary metabolites in Gymnema sylvestre , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture
- E.P. Venkatasalam, Sumita Sharma, K.K. Pandey, Shilpa Sharma, Vandana Thakur, Richa Sood, B.P. Singh, Influence of hermetic and non-hermetic culture rooms on biochemical and in vitro morphological characters of potato cultivars , Indian Journal of Horticulture: Vol. 72 No. 03 (2015): Indian Journal of Horticulture
- Bhupendra Sagore, Kanhaiya Singh, Jai Prakash, Vartika Srivastava, Vignesh M, Brijesh Kumar Yadav, Elucidating the effect of plant bioregulators on embryo maturation for shortening the breeding cycle in papaya , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- B.L. Attri, Hare Krishna, B. Das, J.K. Ranjan, Pragya ., N. Ahmed, Effect of salicylic acid and calcium on the shelf-life of peach cultivars , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- S.K. Bairwa, M.K. Tripathi, S.S. Kushwah, B.S. Baghel, S. Tiwari, Somatic embryogenesis and plantlet regeneration from embryogenic suspension culture in muskmelon , Indian Journal of Horticulture: Vol. 69 No. 03 (2012): Indian Journal of Horticulture
- Anuradha ., S.K. Sehrawat, D.S. Dahiya, Assessment of genetic integrity of plants regenerated by organogenesis from callus culture of strawberry , Indian Journal of Horticulture: Vol. 74 No. 01 (2017): Indian Journal of Horticulture
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Jayoti Majumder, Kanwar P. Singh, S.K. Singh, K.V. Prasad, Manjusha Verma, In vitro morphogenesis in marigold using shoot tip as explant , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- Poonam Kumari, D.V.S. Raju, Kanwar Pal Singh, K.V. Prasad, Sapna Panwar, Characterization of phenolic compounds in petal extracts of rose , Indian Journal of Horticulture: Vol. 75 No. 02 (2018): Indian Journal of Horticulture
- Sapna Panwar, Kanwar Pal Singh, Namita ., T. Janakiram, H. Sonah, T.R. Sharma, DNA fingerprinting in African marigold (Tagetes erecta L.) genotypes using ISSR and URP markers , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Sahar Mirzaei, S.K. Singh, K.P. Singh, K.V. Prasad, S.S. Sindhu, In vitro micropropagation of miniature rose cv. Candy Sunblaze , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- Madhu Bala, Kanwar Pal Singh, S.K. Singh, K.V. Prasad, Standardization of an efficient protocol for in vitro mass multiplication of Hybrid Tea rose cv. Raktima , Indian Journal of Horticulture: Vol. 70 No. 03 (2013): Indian Journal of Horticulture
- Mohan Ram, K.V. Prasad, T. Janakiram, S.K. Singh, Ajay Arora, Callus induction and proliferation from Rosa hybrida leaf explants , Indian Journal of Horticulture: Vol. 72 No. 03 (2015): Indian Journal of Horticulture
- D.V.S. Raju, K.V. Prasad, Namita ., Kanwar Pal Singh, T. Janakiram, A. Nagaraja, Genotypic variation in hip traits of Indian rose varieties , Indian Journal of Horticulture: Vol. 73 No. 03 (2016): Indian Journal of Horticulture
- Arvind K. Verma, K.V. Prasad, S.K. Singh, Surendra Kumar, In vitro isolation of red coloured mutant from chimeric ray florets of chrysanthemum induced by gamma-ray , Indian Journal of Horticulture: Vol. 69 No. 04 (2012): Indian Journal of Horticulture
- M. Lakshmipathy, K.V. Prasad, K.M. Manjaiah, T. Janakiram, Namita ., Performance of turfgrasses on a clay based simulated cricket pitch under varied levels of soil compaction and ABA , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- H.R. Mahure, M.L. Choudhary, K.V. Prasad, S.K. Singh, Mutation in chrysanthemum through gamma irradiation , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture