Genetic diversity analysis of indigenous and exotic apple genotypes using inter simple sequence repeat markers
Downloads
Published
Keywords:
Apple, ISSR-PCR, DNA fingerprinting, genetic diversity, cluster analysisIssue
Section
License
Copyright (c) 2010 Indian Journal of Horticulture

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
The domesticated apple (Malus x domestica Borkh) is one of the most widely cultivated fruit crop of the temperate world. Large number of commercial cultivars and varieties are available which need an accurate characterization to be used in the breeding programmes. Keeping this thing in mind, the genetic diversity among 46 genotypes was investigated using Inter Simple Sequence Repeat (ISSR) markers. A total of 130 amplification products were detected with 21 ISSR primers of which 130 (100%) were reported to be polymorphic. Primers based on (AC)n and (AG)n repeats produced more polymorphic bands. The number of bands generated by 21 ISSR primers ranged from 4(UBC 840) to 10 (ISSR 3) with a mean of 6.19 bands per primer. The band size ranged from 300-1700bp. The Jaccard’s similarity coefficient ranged from 0.435 to 0.822 suggesting that the 46 genotypes used in the study were highly divergent. Cluster analysis based on the UPGMA method and bootstrap analysis separated all the genotypes into three major clusters. Maximum Resolving Power (Rp) was computed to be 5.696 (ISSR 3) while the minimum was 1.174 (UBC 850). The highest Polymorphism Information Content (PIC) was recorded to be 3.946 (ISSR 3) while the lowest was 1.700 (UBC 850). The overall Heterozygosity Averages (Hav) values of primers were 0.068 and 0.717 respectively for ISSR and UBC series. The present study has successfully distinguished morphologically similar genotypes that emphasize the use of molecular markers to the taxonomists.
How to Cite
Downloads
Similar Articles
- P.J. Mishra, B.B. Behera, S. Behera, Carbon sequestration, soil health improvement and livelihood security through mango-based agroforestry system , Indian Journal of Horticulture: Vol. 77 No. 02 (2020): Indian Journal of Horticulture
- Masood Ahmad, Pramod Kumar, Uday Sharma, Pratibha Chib, Generative traits of litchi (Litchi × chinensis Sonn.) cultivars in Shiwalik foothills of North-West Himalayas , Indian Journal of Horticulture: Vol. 81 No. 02 (2024): Indian Journal of Horticulture
- P.K. Shrivastava, B.N. Patel, S.N. Patel, Pitcher irrigation for young mango plantation in water scarce hilly tracts of southern Gujarat , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- J.K. Ranjan, A.K. Chakrabarti, S.K. Singh, Pragya ., Studies on in vitro seed germination of chilli , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- Sheikh Mehraj, A. H. Pandit, K. M. Bhat, S. A. Bhat, M. T. Ali, H. A. Malik, I. A. Bisati, Hilling media influence on clonal propagation of apple rootstocks through layering , Indian Journal of Horticulture: Vol. 77 No. 03 (2020): Indian Journal of Horticulture
- Imtiyaz, T. Nazki, M.A.A. Siddique, Z.A. Rather, Nelofar ., Studies on 60Co gamma irradiation for inducing in vitro mutagenesis in gerbera , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- Rajender Kumar, Variations in physico-chemical traits of tamarind genotypes , Indian Journal of Horticulture: Vol. 78 No. 4 (2021): Indian Journal of Horticulture
- Brij Bihari Sharma, Pritam Kalia, Shrawan Singh, Bhopal Singh Tomar, Dinesh Singh, Race-specific host-plant resistance against black rot (Xanthomonas campestris pv. campestris) in alien Brassicas , Indian Journal of Horticulture: Vol. 78 No. 4 (2021): Indian Journal of Horticulture
- Jayashree E., Shakkira P. K, Anees K, Turmeric press residue – a high-value by-product of turmeric juice powder , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Gulab Choudhary, L. N. Bairwa, O.P. Garhwal, S. K. Bairwa, Manju Netwal, P. K. Kaswan, M. L. Chopra, Rajesh Choudhary, Effect of transplanting dates, cultivars and zinc on yield and economics of onion in semi-arid conditions in Rajasthan , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
<< < 40 41 42 43 44 45 46 47 48 49 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Anjali Soni, Jai Prakash, S.K. Singh, A.K. Goswami, N.C. Gupta, A.K. Singh, Efficacy of gene-based markers associated with sex expression in papaya , Indian Journal of Horticulture: Vol. 74 No. 03 (2017): Indian Journal of Horticulture
- V.S. Meena, E. Nambi, Poonam Kashyap, K.K. Meena, Naphthalene acetic acid and ferrous sulphate induced changes in physico-chemical composition and shelf-life of ber , Indian Journal of Horticulture: Vol. 70 No. 01 (2013): Indian Journal of Horticulture
- M.K. Verma, S.K. Singh, Jai Prakash, A.K. Singh, S.K. Jha, Mechanical behaviour of seed hardness in relation to physico-chemical composition of grape , Indian Journal of Horticulture: Vol. 71 No. 4 (2014): Indian Journal of Horticulture
- S.Y. Maraga, A.K. Singh, T.K. Behera, A.D. Munshi, Sukanta Dash, N. Pachauri, Effect of planting time and fertilizer dose on growth, yield and quality of bitter gourd grown under polyhouse and net house conditions , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- Sanjay K. Singh, R.S. Singh, O.P. Awasthi, Influence of pre- and post-harvest treatments on shelf-life and quality attributes of ber fruits , Indian Journal of Horticulture: Vol. 70 No. 04 (2013): Indian Journal of Horticulture
- Deepa Samant, A.K. Singh, Manish Srivastav, N.K. Singh, Assessment of genetic diversity in mango using inter-simple sequence repeat markers , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- J.S. Bisen, A.K. Singh, Impact of inorganic to organic cultivation practices on yield of tea in Darjeeling hills - A case study , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- Ankit Singh, Manish Srivastav, A.K. Singh, A.K. Dubey, S.K. Lal, Flowering attributes of parental mango genotypes , Indian Journal of Horticulture: Vol. 71 No. 4 (2014): Indian Journal of Horticulture
- A.K. Singh, Ingita Gohain, S. Shyamalamma, Morphological variability in jackfruit grown under agro-forestry system of Tripura , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- Jai Prakash, Kanhaiya Singh, A.K. Goswami, A.K. Singh, Comparison of plant growth, yield, fruit quality and biotic stress incidence in papaya var. Pusa Nanha under polyhouse and open field conditions , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture