Influence of explant collection period, antibrowning strategy and growth regulators composition on in vitro propagation of Bhagwa pomegranate
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2019.00042.2Keywords:
Punica granatum, explant establishment, In vitro, plantlet regenerationIssue
Section
License
Copyright (c) 2019 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
In vitro propagation is a rapid mean for production of disease-free and healthy plants. Using nodal segments for in vitro propagation of pomegranate cv. Bhagwa, the effect of time of explant collection and anti-browning strategy on culture establishment were investigated. A combination of growth regulators (BAP, NAA) and growth substances (AgNO3, Adenine sulphate) were also tested for shoot bud initiation, shoot proliferation and in vitro rooting. In a culture cycle of April to July months, the maximum explant establishment (58.07%) was recorded in the month of April. The medium supplemented with activated charcoal (2.5 g l-1) induced significantly higher culture establishment (63.9%) than control and the treatments involving pre-culture shaking of explants with polyvinylpyrrolidone (PVP) (1.0%) solution or antioxidants (100 mg l-1 citric acid + 150 mg l-1 ascorbic acid) solution. For shoot bud initiation from nodal segments, different levels of BAP (0.5, 1.0, 1.5 and 2.0 mg l-1) alone or with NAA (0.25 mg l‑1) were tested. The significantly highest response (85.17%) was achieved on MS medium supplemented with BAP (1.0 mg l-1) + NAA (0.25 mg l-1). The AgNO3 (1.5 mg l-1) enabled maximum survival of cultured shoots during sub-culture stage. The MS medium containing BAP (0.5 mg l-1) + NAA (0.5 mg l-1) + AgNO3 (1.5 mg l-1) + Adenine sulphate (40 mg l-1) was good for shoot multiplication. The half strength MS medium supplemented with NAA (1.0 mg l-1), was suitable for rooting of shoots in vitro
How to Cite
Downloads
Similar Articles
- A. Chauhan, M. Modgil, Phytotoxic effects of different antibiotics on leaf explants and their potential as Agrobacterium counter selection agents during gene transfer in apple , Indian Journal of Horticulture: Vol. 73 No. 02 (2016): Indian Journal of Horticulture
- Sujata Upadhyay, M.M. Syamal, Hamidullah Itoo, Micropropagation of sweet orange cv. Mosambi through shoot tips and nodal segments , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- E. Asadi, A. Mohammadi Ghehsareh, E. Ganji Moghadam, M. Hodaji, H.R. Zabihi, Improving of pomegranate aril paleness disorder through application of Fe and Zn elements , Indian Journal of Horticulture: Vol. 76 No. 02 (2019): Indian Journal of Horticulture
- P. Jayaprakash, Sheeba D, Vikas ., V. K., Sivasamy M, T. Sabesan, Development of pollen germination medium to test pollen viability of eggplant and its wild species , Indian Journal of Horticulture: Vol. 75 No. 02 (2018): Indian Journal of Horticulture
- Vitthal N. Bhosale, Krishan P. Singh, A.K. Singh, R. Jyothi, Study on rapid mass multiplication of orchid by using immature seeds , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- Ratna Rai, Divyangana, Ranjan Srivastava, Rajesh Kumar, V.P. Singh, Pratibha, An efficient protocol for shoot organogenesis and plant regeneration in jackfruit , Indian Journal of Horticulture: Vol. 79 No. 3 (2022): Indian Journal of Horticulture
- A. Nagaraja, K. Usha, Bhupinder Singh, S.K. Singh, C. Umamaheswari, Effect of temperature and relative humidity on growth and sporulation of Fusarium mangiferae under in vitro conditions , Indian Journal of Horticulture: Vol. 68 No. 01 (2011): Indian Journal of Horticulture
- Resmi Paul, M.R. Shylaja, Preliminary screening of in vitro raised ginger regenerants to soft rot and bacterial wilt diseases using electrolyte leakage method , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- Gajanan Gundewadi, Shalini Gaur Rudra, Robin Gogoi, Haritha Bollinedi, S.K. Singh, R.R. Sharma, In vitro efficacy of essential oils against Colletotrichum gloeosporioides, the causal agent of Anthracnose , Indian Journal of Horticulture: Vol. 79 No. 1 (2022): Indian Journal of Horticulture
- B Zhao, Effect of temperature and GA3 on seed germination and seedling establishment of Rhododendron purdomii Rehd. et Wils , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
<< < 6 7 8 9 10 11 12 13 14 15 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Harsimran Kamboj, Krishan Kumar, J. S. Brar, Anil Kumar, P. K Arora, Optimization of in vitro propagation of jamun variety Konkan Bahadoli , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- Harsimran Singh, Anita Arora, Krishan Kumar, Preliminary screening of citrus hybrids for identifying tolerance to Phytophthora nicotianae , Indian Journal of Horticulture: Vol. 81 No. 01 (2024): Indian Journal of Horticulture
- Anil Kumar, Shashi Pathania, P.K. Arora, Rootstock evaluation for sweet orange cv. Early Gold in arid irrigated region of Punjab , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Mandeep Pathania, Jagdish Kumar Arora, Parshotam Kumar Arora, Incidence and severity of insect-pests and diseases of Kinnow mandarin , Indian Journal of Horticulture: Vol. 78 No. 03 (2021): Indian Journal of Horticulture
- Arun Kishor, Raj Narayan, Manoj Brijwal, Brij Lal Attri, Anil Kumar, Sovan Debnath, Storage behaviour of apple cultivars under ambient conditions , Indian Journal of Horticulture: Vol. 75 No. 02 (2018): Indian Journal of Horticulture
- Anil Kumar, Navin Singh, K.K. Misra, Vishal Nirgude, Effect of foliar spray of calcium chloride and boric acid on shelf-life of guava , Indian Journal of Horticulture: Vol. 74 No. 04 (2017): Indian Journal of Horticulture
- S.S. Manhas, B.S. Gill, Sushil Sharma, Krishan Kumar, Effect of different planting material, planting dates and harvesting dates on growth, yield and quality of turmeric , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture
- B. L. Attri, M. S. Mer, Arun Kishor, Anil Kumar, Shelf life and quality of apple at ambient conditions affected by different concentrations of Aloe vera gel and neem oil , Indian Journal of Horticulture: Vol. 75 No. 04 (2018): Indian Journal of Horticulture
- A. Kishor, Raj Narayan, Manoj Brijwal, B.L. Attri, Anil Kumar, Sovan Debnath, Performance of some new apple cultivars for yield and physico-chemical characters under mid-hill conditions of Uttarakhand , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Anil Kumar, Shashi Pathania, Parshotam Kumar Arora, Performance of early ripened varieties of ‘Sweet Orange’ in arid irrigated region of Punjab , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture