Utilization of banana peel for pectinase enzyme production
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Musa sp. Banana peel, pectinase production, optimization, A. nigerIssue
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Banana peel, a fruit waste rich in pectin and other nutrients, was utilized in the present study to produce an industrially important pectinase enzyme. Banana peel powder as a sole source of carbon has been successfully exploited to grow Aspergilus niger (MTCC 281) under solid-state fermentation (SSF). The effect of quantity of nutrients, pH, temperature, and dilution level was studied on pectinase production. Optimizing the conditions for the production of strains and enzymes is necessary to use agricultural waste and properly attain sustainability of the bioprocess. Maximum pectinase activity of 8.12U/ml was observed at 1:3 dilution levels, pH 5, and 3% of 108 spores/ml inoculums size at 40°C with 120 hrs of incubation time. The current study addresses optimizing different cultural conditions for A. niger to maximize pectinase production with cheap and readily available banana peeling waste.Abstract
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Ahmed, I., Zia, M.A., Hussain, M.A., Akram, Z., Naveed, M.T. and Nowrouzi, A. 2016. Bioprocessing of citrus waste peel for induced pectinase production by Aspergillus niger; its purification and characterization. J. Radiat. Res. Appl. Sci. 9: 148-54. Barman, S., Sit, N., Badwaik, L.S. and Deka, S.C. 2015. Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice. J. Food Sci. Technol. 52: 3579- Dhillon, S.S., Gill, R.K., Gill, S.S. and Singh, M. Studies on the utilization of citrus peel for pectinase production using fungus Aspergillus niger. Int. J. Environ. Stud. 61: 199-210 Iqbal, H.M.N., Asgher, M. and Bhatti, H.N. 2011. Optimization of physical and nutritional factors for synthesis of lignin degrading enzymes by a novel strain of Trametes versicolor. Bioresources : 1273-87. Koser, S., Anwar, Z., Iqbal, Z., Anjum, A., Aqil, T., Mehmood, S. and Irshad, M. 2014. Utilization of Aspergillus oryzae to produce pectin lyase from various agro-industrial residues. J. Radiat. Res. Appl. Sci. 7: 327-32. Maller, A., Damasio, A.R.L., Silva, T.M., Jorge, J.A., Terenzi, H.F. and Polizeli, M.L.T.M. 2011. Biotechnological potential of agro-industrial wastes as a carbon source to thermostable polygalacturonase production in Aspergillus niveus. Enzyme Res. 2011 Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-28. Patil, S.R. and Dayanand, A. 2006. Production of pectinase from deseeded sunflower head by Aspergillus niger in submerged and solid-state conditions. Bioresour. Technol. 97: 2054-58. Reginatto, C., Rossi, C., Miglioranza, B.G., dos Santos, M., Meneghel, L., da Silveira, M.M. and Malvessi, E. 2017. Pectinase production by Aspergillus niger LB-02-SF is influenced by the culture medium composition and the addition of the enzyme inducer after biomass growth. Process Biochem. 58: 1-8. Salim, D., Anwar, Z., Zafar, M., Anjum, A., Bhatti, K.H. and Irshad, M. 2018. Pectinolytic cocktail: Induced yield and its exploitation for lignocellulosic materials saccharification and fruit juice clarification. Food Biosci. 22: 154-64. Sethi, B. K., Nanda, P. K. and Sahoo, S. 2016. Characterization of biotechnologically relevant extracellular lipase produced by Aspergillus terreus NCFT 4269.10. Braz. J. Microbiol. 47: -49. Thangaratham, T. and Manimegalai, G. 2014. Optimization and production of pectinase using agro waste by solid state and submerged fermentation. Int. J. Curr. Microbiol. Appl. Sci. 3: –65. Tibolla, H., Pelissari, F. M., Martins, J. T., Vicente, A. A. and Menegalli, F.C. 2018. Cellulose nanofibers produced from banana peel by chemical and mechanical treatments: characterization and cytotoxicity assessment. Food Hydrocoll. 75: 192-201. Zaini, H.B.M., Sintang, M.D.B. and Pindi, W. 2020. The roles of banana peel powders to444444 alter technological functionality, sensory and nutritional quality of chicken sausage. Food Sci. Nutr. 8: 5497-5507.
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