Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine

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Published

2023-12-30

DOI:

https://doi.org/10.58993/ijh/2023.80.4.2

Keywords:

Banana, Radiation, Mutation, In vitro, LD50
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Issue

Vol. 80 No. 04 (2023): Indian Journal of Horticulture

Section

Articles

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Copyright (c) 2023 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Rajni Rajan Bihar Agricultural University, Sabour 813 210, Bihar, India
  • Muhammad Feza Ahmad Bihar Agricultural University, Sabour 813 210, Bihar, India
  • Awadhesh K. Pal Bihar Agricultural University, Sabour 813 210, Bihar, India
  • Kuldeep Pandey Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh https://orcid.org/0000-0001-7347-5232

Abstract

An investigation was carried out to initiate the in vitro mutagenesis in banana cv. Grand Naine using shoot
tip culture. Based on the sensitivity of explants, the lethal dose was estimated using probit analysis. As a result
of the adverse effects of higher irradiation doses, it was decided that determining the lethal dose can be very
beneficial in reducing population loss. The LD50 dose of gamma irradiation of the banana shoot tip culture was
determined to be 39.5 Gy. The study also discovered that 13 DAS explants when treated with 10 Gy recovered
the highest number of shoots. The optimization of gamma irradiation with subculture days in explants of banana
may be effective in developing significant variations with a particular trait of interest

How to Cite

Rajan, R., Ahmad, M. F., Pal, A. K., & Pandey, K. (2023). Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine. Indian Journal of Horticulture, 80(04), 313–318. https://doi.org/10.58993/ijh/2023.80.4.2

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