Establishment of in vitro propagation protocol for Hybrid Tea rose cv. Raktagandha

Published

2017-06-13

DOI:

https://doi.org/10.5958/0974-0112.2017.000050.0

Keywords:

Hybrid tea, micropropagation, protocol, rose.
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Authors

  • Shyama Kumari Department of Horticulture (Vegetable and Floriculture), Bihar Agricultural College, Sabour, Bhagalpur 813 210
  • Kanwar Pal Singh Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • S.K. Singh Division of Fruits and Horticultural Technology, ICAR-IARI, New Delhi
  • Surendra Kumar Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • Subhashish Sarkhel Department of Plant Pathology, Bihar Agricultural College, Sabour, Bhagalpur

Abstract

An efficient protocol for in vitro multiplication of hybrid tea rose cv. Raktagandha was developed using axillary bud segments. Out of different pre-treatments of explants tried, the highest explant survival (78.05%) was obtained with carbendazim (0.2%) + diathane M-45 (0.2%) + 8-HQC (200 mg/l) for 3 h on a horizontal shaker (120 rpm). Sucrose concentration of 30 g/l in the medium seems to be optimal for in vitro shoot multiplication. Murashige and Skoog medium supplemented with 3.5 mg/l BAP + 0.2 mg/l NAA + 0.5 mg/l GA3 was found most effective for culture establishment and shoot proliferation with highest number of micro-shoots (4.62 shoots / explant). Rooting of micro-shoots was induced on half-strength MS basal medium supplemented with NAA (0.5 mg/l) + IBA (0.5 mg/l). The regenerated plantlets were efficiently hardened in glass jars filled with vermiculite + agropeat (1:2) moistened with half-strength MS medium salts and covered with polypropylene lids, thereafter plants were successfully transferred to the glasshouse with good survival.

How to Cite

Kumari, S., Singh, K. P., Singh, S., Kumar, S., & Sarkhel, S. (2017). Establishment of in vitro propagation protocol for Hybrid Tea rose cv. Raktagandha. Indian Journal of Horticulture, 74(02), 245–250. https://doi.org/10.5958/0974-0112.2017.000050.0

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