Development of an efficient micropropagation technique for Musa cv. Udhayam (ABB)

Published

2014-12-25

Keywords:

Banana, recalcitrantce, Pisang Awak, micropropagation, shoot tip, direct organogenesis
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Authors

  • M.S. Saraswathi ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu
  • S. Praveena ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu
  • S. Uma ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu
  • R. Thangavelu ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu
  • G. Kannan ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu
  • S. Backiyarani ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu
  • T. Arivazhagan ICAR-National Research Centre for Banana, Thogamalai Road, Thayanur Post, Tiruchirappalli 620 102, Tamil Nadu

Abstract

Experiments were conducted to devise an efficient method of micropropagation for a high yielding but recalcitrant banana cv. Udhayam (Pisang Awak, ABB) using shoot tip explants. Virus-indexed shoot tips were established in medium comprising full-strength Murashige and Skoog (MS) basal salts and vitamins, supplemented with 50 mg l-1 ascorbic acid, 100 mg l-1 myo-inositol and 4.0 mg l-1 benzylamino purine (BAP). Among the various media tested for shoot proliferation, MS medium with BAP (3.0 mg l-1) and 5% coconut water (CW) was found optimum as it produced the maximum number of 6.3 multiple shoots in a minimum period of 7.5 days. Rooting was achieved in the MS medium fortified with indole butyric acid (IBA) 0.5 mg l-1 + naphthalene acetic acid (NAA) 1.0 mg l-1 + activated charcoal (AC) 250 mg l-1. Fully hardened planting material ensured high survival upon field transplantation. The development of a complete tissue culture protocol also includes the optimization of media and environment for successful primary and secondary hardening which has also been accomplished in the study.

How to Cite

Saraswathi, M., Praveena, S., Uma, S., Thangavelu, R., Kannan, G., Backiyarani, S., & Arivazhagan, T. (2014). Development of an efficient micropropagation technique for Musa cv. Udhayam (ABB). Indian Journal of Horticulture, 71(4), 452–457. Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/1373

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