Genetic diversity and relationship study of single and double petal tuberose (Polianthes tuberosa L.) cultivars based on RAPD and ISSR markers

Abstract

Tuberose (Polianthes tuberosa L.) is a commercial ornamental bulbous crop with highly fragrant flowers. In the present study, genetic diversity among 12 tuberose cultivars were studied using randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Altogether 86 amplicons were amplified with 16 selected RAPD primers out of which, 70 (81.39%) were polymorphic with an average of 4.37 amplicons per primer. Similarly, nine ISSR primers amplified a total of 40 scorable amplicons with sizes ranging between 100-3000 bp. Out of 40 ISSR amplicons, 37 (92.50%) were polymorphic, with an average of 4.1 amplicons per primer. RAPD markers based analysis grouped 12 cultivars into two clusters, whereas ISSR markers based analysis grouped 12 cultivars in three clusters. Analysis of molecular variance (AMOVA) study showed only 5% of variation between single and double petal population, whereas, greater variation (95%) was observed only among the cultivars of each petal type. Principal Coordinate Analysis (PCoA) showed the percentage of variation explained by the first 3 axes was 51.44%.